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virology blog

virology blog

About viruses and viral disease

Virology Toolbox

Methods used for the study of viruses

Viral growth

Multiplicity of Infection
Influenza virus growth in eggs

Viral detection
Influenza hemagglutination inhibition assay
Influenza microneutralization assay
Detecting viruses: the plaque assay
Measurement of viruses by end-point dilution assay
The western blot
Detection of antigens or antibodies by ELISA
Detecting viral proteins in infected cells or tissue by immunostaining

Viral Bioinformatics
Introduction + Homology
Dotplot
Recombination
Introduction to multiple sequence alignment
Viral bioinformatics: Multiple sequence alignment – Jalview
Viral bioinformatics: Multiple sequence alignment – Base-By-Base (BBB) editor
Viral bioinformatics: Sequence searcher

Reader Interactions

Comments

  1. Thor says

    22 July 2010 at 10:01 pm

    This page is an excellent idea.

  2. Daniel says

    27 July 2010 at 9:43 pm

    This is a great idea and great help for starters at virological field!

  3. rashid4viroogy says

    20 August 2011 at 11:31 am

    Great info sir..

  4. Sgalattas says

    7 October 2011 at 7:13 pm

    Hello Dr., I need to know how did u calculate the equation 4 the plaque assayplz?

  5. omar alshmari says

    18 December 2011 at 4:58 pm

    hi

  6. omar alshmari says

    18 December 2011 at 5:00 pm

    are there any videos about virus vs. bacteria

  7. mariya says

    6 May 2012 at 11:14 am

    hello,
    i would like to know how to detect the antiviral activity?

  8. Masud1234 says

    16 May 2012 at 12:31 pm

    I do have a practical problem. I measured TCID50 value of my virus (1.6X10^4/ml) now like to use it as 1 TCID50 or 100 TCID50. My final volume of infection is 100 microliter. How much virus I need to add from the above stock. Does the number of cell(s) in the culture influence the calculation. Please discuss anybody who ever interested. Thanks.
    Khan

  9. flu_knerd says

    2 November 2012 at 4:01 pm

    Do you have recommendations or resources for protocols (books or online)? I am also looking for a resource to learn about the cell lines and strains commonly used in virology research.

  10. dhanya says

    28 May 2013 at 7:24 am

    great work!!! love this blog always 🙂

  11. Jeff Taylor says

    8 January 2015 at 11:44 am

    Hi:

    Let me present a pre-clinical animal model using
    primates. I don’t know if you have any exposure but it would lead to
    further clinical trials which could be strategic in your region. Please
    see the following message:

    Do you have capabilities for. a pre-clinical study, to help
    in an Ebola study using primates?

    The design is very simple.

    We were told that most trials have been using 4
    primates. The primates are exposed to Ebola, then the test articles are
    administered and survivability is noted.

    In this case we want to test as a prophylactic. That
    would mean administering the test article to the group, then exposing them to
    Ebola.

    Very frankly I am not certain on the controls. Two
    controls, two with prophylactic?

    We need to talk to the P.I.

    Our test article is based on a formula we used a few years
    back that was successful with HIV. We understand the Ebola virus and have
    modified the formula through our in silico process. We are in the middle
    of the in vitro assays which will be finished mid February and must prepare to
    be ready for the NHP phase. We need a door opened at a level 4 laboratory
    that handles Ebola. jtaylor@nwclinicalresearch.com

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by Vincent Racaniello

Earth’s virology Professor
Questions? virology@virology.ws

With David Tuller and
Gertrud U. Rey

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Inside a BSL-4
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