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About viruses and viral disease

The Wall of Polio, version 3.0

2 July 2015 by Vincent Racaniello

Wall of Polio 3.0Back in 2013 I built a Wall of Polio in my laboratory – a large stack of six-well cell culture plates that have been used to measure the concentration of polioviruses in various samples by plaque assay. It became a focal point of the lab at which many guests came to have their photographs taken. Sadly, the Wall fell twice. Now a new Wall – version 3.0 – has been completed.

The new Wall of Polio is in my office at Columbia University Medical Center, where it will not annoy the Fire Inspector (the former Wall partially blocked an aisle). Furthemore, the new Wall is glued together, so it will not come apart. Its construction is documented in the photographs below. The Wall of Polio 3.0 is built with 1,464 six-well plates of HeLa cells that were used to determine the titer of poliovirus. We have also already had a number of visitors to Wall 3.0.

Because the Wall is impressive, it attracts attention, which can then be used to explain the plaque assay and determining virus titer. Therefore it is simply another tool that I used to teach the world about virology.

When you visit, expect that I will ask to photograph you before the Wall. Only a few have refused.

Wall of Polio
Starting to rebuild the wall, with glue, in my office. 8 January 2015.
Wall of Polio
27 February 2015.
Wall of Polio
The Wall grows. 28 January 2015.
Emily
Emily (Wall rebuild in progress)
Wondering how to get around light switch and thermostat.
Built around light switch.
Jennifer McLennan, eLife
Built around thermostat.
Robert Demarest, artist
Laura
Xuanyi #2
Nada
Herminio
Justina
Vincent
Monique
Rob Verger
Olumolade
Me
Monique #2
Amy
Samantha
Julio Ruiz-Saenz and family
Emma

 

Filed Under: Basic virology, Information Tagged With: cell culture, HeLa cells, plaque assay, poliovirus, titer, titration, viral, virology, virus, wall of polio

Reader Interactions

Comments

  1. Sammie Didi says

    3 July 2015 at 2:07 am

    Also love the reflection of the window city 😉

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by Vincent Racaniello

Earth’s virology Professor
Questions? virology@virology.ws

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Contents

Table of Contents
ME/CFS
Inside a BSL-4
The Wall of Polio
Microbe Art
Interviews With Virologists

Earth’s Virology Course

Virology Live
Columbia U
Virologia en Español
Virology 101
Influenza 101

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This Week in Microbiology
This Week in Parasitism
This Week in Evolution
Immune
This Week in Neuroscience
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Polio eradication
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Small Things Considered
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The Living River
Parasites Without Borders

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