The Polio Wall of FameÂ (pictured) is a set of fifteen sculptured busts of 17 individuals who made important contributions to understanding and preventing poliomyelitis. The busts are mounted on an exterior wall of Founder’s Hall at theÂ Roosevelt Warm Springs Institute for Rehabilitation in Warm Springs, Georgia, USA.
In my laboratory we have a slightly different wall – we call it The Wall of Polio. It consists of a collection of six-well cell culture plates that have been used to measure the concentration of polioviruses in various samples by plaque assay.
The plaque assay isÂ one of the most important procedures in virology for measuring the virus titer â€“ the concentration of viruses in a sample. This technique was first developed to calculate the titers of bacteriophage stocks.Â Renato DulbeccoÂ modified this procedure in 1952 for use in animal virology, and it has since been used for reliable determination of the titers of many different viruses.
We love the plaque assay so much that we cannot bear to throw away the plates after they have been counted. They reside in various nooks and crannies in the laboratory, but one creative use has been to construct a wall – think of Legos using cell culture plates. When a visitor comes to the lab, I photograph them in front of the Wall of Polio (sign inspired by Pink Floyd). When you visit don’t be surprised when I ask to photograph you in front of the Wall of Polio.
Update from my postdoctoral scientist Rea:Â The wall has >1000 plates, stands over 6 ft tall, and contains data from only one experiment which took me almost 4 months to do. Credit goes toÂ Brenda RaudÂ for the sign, construction and design, and some plates too!