A viral protease interferes with interferon

15 June 2009

Viruses are known to evade the early, or innate, host defenses by interfering with the sensing of infection, production of IFN, and synthesis and activity of ISGs. Today we’ll examine the evidence that the function of one or more ISGs is blocked in poliovirus-infected cells.

When IFN is added to the medium of cultured cells, hundreds of ISGs are produced, establishing an antiviral state. Many viruses are unable to replicate in cells treated in this way. An example is the inhibition of replication of encephalomyocarditis virus (EMCV), a picornavirus, by IFN shown in this figure:

emcv-ifn

In this experiment, IFN was added to the medium of HeLa cells for 16 hours, and then the cells were infected with EMCV. At the indicated times after infection, the medium was removed, and EMCV titer was determined by plaque assay. In mock-treated cells, EMCV produces about 100 plaque-forming units (pfu) per cell*, while IFN treatment of cells blocks virus production.

If the same experiment is done using poliovirus, the outcome is quite different. As shown in the figure below, poliovirus replication is reduced by IFN treatment of cells, but a good amount of virus is nevertheless produced.

polio-ifn

This result suggests that the genome of poliovirus encodes one or more proteins that antagonize the activity of ISGs. Likely candidates for the ISG-busting activity are the two proteases encoded in the viral genome. These proteases, called 2Apro and 3Cpro, process the viral protein and also are known to inactivate cellular proteins. Consistent with this idea, poliovirus with a one amino acid change in 2Apro was found to be completely sensitive to IFN.

This observation indicates that 2Apro blocks the antiviral activity of one or more ISGs.

The EMCV genome encodes a 2A protein that does not have the ability to cleave other proteins. This observation may explain the exquisite sensitivity of EMCV to IFN. Can poliovirus 2Apro turn EMCV into an IFN-resistant virus? To test this hypothesis, the gene encoding 2A was inserted into the genome of EMCV. The resulting virus, EMCV-2A, was able to replicate in cells treated with IFN.

Which ISGs are targeted by poliovirus 2Apro? That is not an easy question to answer, as there are nearly 1000 known ISGs. We’re working on it.

(*The plaque assay is one of several methods for determining virus titer. We’ll discuss this assay in an upcoming post).

Morrison, J., & Racaniello, V. (2009). Proteinase 2Apro Is Essential for Enterovirus Replication in Type I Interferon-Treated Cells Journal of Virology, 83 (9), 4412-4422 DOI: 10.1128/JVI.02177-08

  • thor183

    One thing I'm curious about – You've talked about the variability of viruses like the Influenza viruses and some of the consequences. I'm curious about the variability of the immune system across humans. I imagine that many of the receptors, ligands, IFNs, ISGs, etc vary from person to person. Would this be right? Are there differences between individuals down at the molecular level in terms of IFNs, receptors, etc?

    If so, how much research had been done on this variability and the role it may play in the virulence of different viral strains?

  • http://www.virology.ws profvrr

    You are correct – the variability in the immune is likely to be a
    significant determinant of disease susceptibility. It probably
    explains why some people who appear healthy develop severe fatal
    complications after influenza. There is a good deal of active research
    in this area. For example, polymorphisms in toll-like receptor 3 is
    known to predispose to herpes simplex encephalitis. A good review is
    Clin Rev Allergy Immunol. 2009 May 9: The Role of Toll-Like Receptor
    Signaling in Human Immunodeficiencies.

  • http://www.virology.ws profvrr

    You are correct – the variability in the immune is likely to be a
    significant determinant of disease susceptibility. It probably
    explains why some people who appear healthy develop severe fatal
    complications after influenza. There is a good deal of active research
    in this area. For example, polymorphisms in toll-like receptor 3 is
    known to predispose to herpes simplex encephalitis. A good review is
    Clin Rev Allergy Immunol. 2009 May 9: The Role of Toll-Like Receptor
    Signaling in Human Immunodeficiencies.