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parvovirus

TWiV 520: This old mouse

18 November 2018 by Vincent Racaniello

The TWiVidae review universal influenza vaccines that are in clinical trials, and discovery of an atypical parvovirus that causes chronic kidney disease in middle aged, immunocompromised laboratory mice.

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Show notes at microbe.tv/twiv

Filed Under: This Week in Virology Tagged With: chronic kidney disease, clinical trial, HA stalk vaccine, immunocompromised, immunosufficient, influenza, kidney fibrosis, parvovirus, peptide, tubulointerstitial nephropathy, universal influenza vaccine, viral, virology, virus, viruses

Leaving Koch behind

15 November 2018 by Vincent Racaniello

parvovirusFor the past 40 years, certain laboratory mice in Australia and the US have been unexpectedly dying in middle age, but the cause has remained elusive. A novel member of the parvovirus family appears to be the culprit.

[Read more…] about Leaving Koch behind

Filed Under: Basic virology, Information Tagged With: chronic kidney disease, inclusion body nephropathy, interstitial fibrosis, intranuclear inclusion bodies, kidney disease, koch's postulates, parvovirus, tubular necrosis, viral, virology, virus, viruses

TWiV 436: Virology above Cayuga’s waters

9 April 2017 by Vincent Racaniello

At Cornell University in Ithaca, New York, Vincent speaks with Susan, Colin, and Gary about the work of their laboratories on parvoviruses, influenza viruses, and coronaviruses that infect dogs, cats, horses and other mammals.

You can find TWiV #436 at microbe.tv/twiv, or listen below.

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Filed Under: This Week in Virology Tagged With: Cornell University, coronavirus, host range, influenza virus, parvovirus, tropism, veterinary medicine, viral, virology, virus, virus entry, viruses

TWiV 361: Zombie viruses on the loose

1 November 2015 by Vincent Racaniello

On episode #361 of the science show This Week in Virology, the TWiVsters discuss Frederick Novy’s return from retirement to recover a lost rat virus, and evidence for persistence of Ebolavirus in semen.

You can find TWiV #361 at www.microbe.tv/twiv.

Filed Under: This Week in Virology Tagged With: bacteriology, ebolavirus, Frederick Novy, Kilham rat virus, meningitis, parvovirus, Paul De Kruif, persistence, semen, sexual transmission, University of Michigan, viral, virology, virus

TWiV 315: Must be something in the water

14 December 2014 by Vincent Racaniello

On episode #315 of the science show This Week in Virology, Vincent, Alan, Rich and Kathy discuss the association of a virus with sea star melting disease, and the finding of a phycodnavirus in the oropharynx of humans with altered cognitive functions.

You can find TWiV #315 at www.microbe.tv/twiv.

Filed Under: This Week in Virology Tagged With: algae, asteroid, chlorovirus, cognitive function, densovirus, oropharynx, Paramecium bursaria, parvovirus, phycodnavirus, sea star, sea star wasting disease, viral, virology, virome, virus

A virus that melts sea stars

17 November 2014 by Vincent Racaniello

Sun flower sea starSea stars are lovely marine invertebrates with a round central body connected to multiple radiating legs (photo credit). In the past year millions of sea stars in the west coast waters of North America have melted into piles of slime and ossicles. Sea star associated densovirus might be the cause of this lethal disease.

Sea star wasting disease (SSWD) is characterized by lesions, limb curling and deflation, and death as the animals rapidly degrade or ‘melt’. The current outbreak began in June 2013 and has killed sea stars from Baja California, Mexico, to Southern Alaska. SSWD might be the biggest marine wildlife epizootic ever observed.

Evidence that SSWD is caused by a virus came from experiments in which extracts of diseased sea stars were passed through a filter with pore sizes small enough to allow passage of viruses but not bacteria or other microbes. When injected into healthy sea stars, these filtrates induced sea star wasting disease. Extracts of diseased sea stars collected in Vancouver, CA contained 25 nanometer virus particles, as determined by electron microscopy.

Nucleic acid sequencing was to identify the viral agent of SSWD. Virus particles were purified from diseased animals, and both DNA and RNA was extracted. Analysis of the nucleotide sequences revealed the presence of giant DNA viruses such as mimiviruses and phycodnaviruses (link to algal virus paper), and among RNA viruses, retroviruses, dicistroviruses, and parvoviruses. With few exceptions, all samples containing parvoviruses were from symptomatic asteroids, and so the authors decided to pursue the study of this virus.

Analysis of the DNA sequence data revealed the presence of a densovirus (a parvovirus) related to viruses found in Hawaiian sea urchins. The authors called this virus sea star-associated densovirus, SSaDV. Like other members of the parvovirus family, these are small (25 nm diameter), naked icosahedral viruses with a ~6 kb single stranded DNA genome. When sea stars were infected in the laboratory with filtrates from diseased animals, virus loads, determined by PCR, increased with time together with disease progression. Field surveys revealed that the virus was more abundant in diseased than in healthy sea stars. The virus was found in marine sediments, plankton, and sea urchins. Viral nucleic acid was also found in sea stars preserved in museums since 1942.

While retrovirus sequences were found in sea star tissues, the authors believe that such ‘Retroviral annotations are likely spurious because they were detected in DNA libraries (and have RNA as nucleic acids).’ The detection of retrovirus sequences in sea stars is not at all spurious! DNA copies of retrovirus genomes are produced during infection and integrated into the host genome, explaining why these sequences were detected in DNA libraries. Their absence in RNA libraries means that virus particles are not produced, as is the case in many other organisms that contain endogenous retroviruses.

The evidence that SSaDV causes sea star wasting disease is strong but not yet complete. The crucial experiment that remains to be done is to isolate infectious virus in cell culture, inoculate it into sea stars, and show that it causes wasting disease.

While the authors’ work reveals that sea star wasting disease virus has been present on the North American Pacific Coast for over 70 years, the disease is not always observed. The current outbreak has been ongoing since just June 2013. Perhaps other environmental conditions have lead to the increased susceptibility of sea stars to disease. It is important to determine if a human activity is involved in precipitating the disease, so that we can prevent future loss of sea stars. The authors suggest one possibility, that increased populations of adult sea stars in small bays and inlets have produced increased virus levels which lead to more infections. It will also be important to determine if the virus has undergone any changes in transmissibility or virulence.

Sea star associated densovirus was also found in non-asteroids, including ophiuroids (brittle stars and basket stars) and echinoids (sea urchins and sand dollars). We need to know the host range of this virus, how it is transmitted, and whether it can cause disease in other species. A troubling scenario is that the recent amplification of the virus in sea stars could lead to infection, and perhaps death of, many other marine species.

Filed Under: Basic virology, Information Tagged With: densovirus, echinoderm, marine invertebrate, parvovirus, Pycnopodia helianthoides, sea star, starfish, viral, virology, virus

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by Vincent Racaniello

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