There is an excellent question in the comments to “Are all virus particles infectious?“: if the particle-to-PFU ratio for a virus stock is 10,000:1, and I infect 1,000,000 cells with 10,000 particles, how many plaques would I expect to observe? Answering this question provides insight into the particle-to-PFU ratio of viruses. If we take 10,000 particles …
Hosts: Vincent Racaniello, Alan Dove, Dickson Despommier, and Rich Condit Vincent, Alan, Dickson and Rich answer listener questions about XMRV, yellow fever vaccine, virus-like particles, West Nile virus, amyotrophic lateral sclerosis and human endogenous retroviruses, multiplicity of infection, and how to make a poxvirus. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV129.mp3″] Click the arrow above to play, or right-click to download …
Hosts: Vincent Racaniello, Alan Dove, and Rich Condit On episode #118 of the podcast This Week in Virology, Vincent, Alan, and Rich answer listener questions about vaccinia virus, fungal viruses, synthetic viruses, influenza vaccine, HeLa cells, multiplicity of infection, and much more. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV118.mp3″] Click the arrow above to play, or right-click to download TWiV #118 (68 …
Chris Upton, a contributor to the virology toolbox, has raised an important point about multiplicity of infection: Perhaps this is a place to bring up particle to pfu ratio? The above is great for when talking about phage, for example, when the ratio approaches 1. But with something like polio when it can be very …
Multiplicity of infection (MOI) is a frequently used term in virology which refers to the number of virions that are added per cell during infection. If one million virions are added to one million cells, the MOI is one. If ten million virions are added, the MOI is ten. Add 100,000 virions, and the MOI …
