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TWiV 588: Coronavirus update – Save the pangolin!

23 February 2020 by Vincent Racaniello

The TWiV team returns this week to SARS-CoV-2019 coverage to review the latest epi curves, the fatality rate, furin cleavage site and receptor binding domain in the spike glycoprotein, related CoV recovered from pangolins, evidence that the virus did not escape from a laboratory, and many more questions sent in by listeners.

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Filed Under: This Week in Virology Tagged With: ACE2, China, coronavirus, CoV, COVID-19, epidemic, evolution, furin, pangolin, pneumonia, receptor binding domain, respiratory aerosol, SARS-CoV-2, spike glycoprotein, viral, virology, virus, viruses, Wuhan, zoonosis

The growing coronavirus epidemic

30 January 2020 by Vincent Racaniello

coronavirusAn unprecedented amount of information is emerging on the new coronavirus, provisionally called 2019-nCoV, that originated in China and is spreading globally. As of this writing there are 8,236 confirmed cases (8,124 in China) with 171 deaths (Click the link for real-time updates).

What was the origin of the virus?

The virus was first isolated by infection of cells in culture with broncho-alveolar wash from a patient in Wuhan with pneumonia. The infected cells showed cytopathic effects, and staining of cells with an antibody to coronavirus NP protein, which is conserved among coronaviruses, revealed intracellular staining.

[Read more…] about The growing coronavirus epidemic

Filed Under: Basic virology, Information Tagged With: 2019-nCoV, bat, China, coronavirus, epidemic, reproductive index, viral, virology, virus, viruses, Wuhan, zoonosis

TWiV 584: Year of the coronavirus

26 January 2020 by Vincent Racaniello

Coronavirus expert Ralph Baric joins TWiV to explain the virology and epidemiology of the recent zoonotic outbreak spreading across China and overseas.

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Filed Under: This Week in Virology Tagged With: bat, China, coronavirus, epidemiology, spillover, travel restriction, viral, virology, virus, viruses, Wuhan, zoonosis

TWiV 582: This little virus went to market

12 January 2020 by Vincent Racaniello

TWiV provides updates on the new coronavirus causing respiratory disease in China, the current influenza season, and the epidemic of African swine fever, including determination of the three-dimensional structure of the virus particle.

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Filed Under: This Week in Virology Tagged With: african swine fever virus, capsid, China, coronavirus, cryo-electron micrography, giant virus, Huanan Fish Market, influenza, influenza excess mortality, influenza like illness, influenza vaccine, pig, swine, three dimensional virus structure, viral, virology, virus, viruses, Wuhan pneumonia, zoonosis

Yet another avian influenza virus, H10N8, infects humans

10 February 2014 by Vincent Racaniello

chicken market

To the collection of avian influenza viruses known to sporadically infect humans – H5N1, H7N9, H7N2, H7N3, H7N7, H9N2, and H10N7 – we can now add H10N8, recently found in two individuals in China.

Avian influenza virus H10N8 was first detected in tracheal aspirates from a 73 year old woman who was hospitalized in November 2013 for severe respiratory illness. The patient, who died, had previously visited a live poultry market. A second infection with this virus was detected in January 2014.

Virus isolated from tracheal aspirates on day 7 of illness was named A/Jiangxi-Donghu/346/2013(H10N8). Nucleotide sequence analysis of the viral genome reveals that it is a reassortant. The HA gene most closely resembles that of a virus isolated from a duck in Hunan in 2012, while the NA gene resembles that of a virus isolated from a mallard in Korea in 2010. All six other RNA segments resemble those from circulating H9N2 viruses in China. These viruses have also provided genes for H7N9 and H5N1 viruses.

Examination of the viral protein sequences provides some clues about virulence of the virus. The HA protein sequence reveals a single basic amino acid at the cleavage site, indicating that the virus is of low pathogenicity in poultry, like H7N9 virus. The sequence in the sialic acid binding pocket of the HA protein indicates a preference for alpha-2,3 linked sialic acids, typical  for avian influenza viruses (human influenza viruses prefer alpha-2,6 linked sialic acids). A lysine at amino acid 627 in the PB2 protein is known to enhance the ability of the virus to replicate at mammalian temperatures; the H10N8 virus has a mixture of lysine and glutamic acid, the residue associated with less efficient replication. The sequence of the M2 protein indicates that the virus is resistant to the antiviral adamantanes. In vitro testing indicated sensitivity to NA inhibitors Tamiflu and Relenza.

It is not known if this novel H10N8 virus will spread further in the human population. A novel influenza H7N9 virus was first detected in humans in early 2013 and has since caused 250 human infections with 70 deaths. Similar incursions of avian influenza viruses into humans have probably taken place for as long as humans have had contact with poultry. We are now adept at detecting viruses and therefore we are noticing these infections more frequently.

Live poultry markets are clearly a risk factor for humans to acquire infections with avian influenza viruses, as noted by Perez and Garcia-Sastre:

Live bird markets in Asia are undoubtedly the major contributor in the evolution of avian influenza viruses with zoonotic potential, a fact for which we seem to remain oblivious.

Given their role in transmitting new viruses from animals to humans, I wonder why live poultry markets are not permanently closed.

Update: George Gao agrees that the live poultry markets in China should be closed.

Filed Under: Basic virology, Information Tagged With: avian influenza, China, H10N8, H5N1, h7n9, HA, NA, viral, virology, virus, zoonosis, zoonotic

Update on influenza H7N9 virus at ASM in Denver

22 May 2013 by Vincent Racaniello

At the 2013 ASM meeting in Denver, Colorado, Stanley Maloy discussed human infections with avian influenza H7N9 virus with Ronald Atlas, Ph.D., University of Louisville, KY; Robert Webster; St. Jude’s Research Hospital, Memphis, TN; Albert Osterhaus; Erasmus Medical Center, Rotterdam, The Netherlands; and Carole Heilman, NIAID, NIH, Bethesda, MD.

Filed Under: Basic virology, Information Tagged With: Albert Osterhaus, avian influenza H7N9, Carole Heilman, China, H2N9, H5N1, pandemic, Robert Webster, Ron Atlas, Stan Maloy, viral, virology, virus

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by Vincent Racaniello

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