by Gertrud U. Rey Antibodies are large proteins that are made by B cells of the adaptive immune system. Most people think that antibodies function only as a whole molecule, but some of the individual fragments of an antibody can also bind and neutralize antigens. An antibody consists of two heavy chains and two light …
Vincent and Alan speak with Erica Ollmann Saphire about her career and her work on understanding the functions of proteins of Ebolaviruses, Marburg virus, and other hemorrhagic fever viruses, at ASM Microbe 2016 in Boston, MA. You can find TWiV #394 at microbe.tv/twiv, or listen or watch the video below. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV394.mp3″] Click arrow to …
On episode #220 of the science show This Week in Virology, Vincent, Rich, Alan, and Kathy discuss regulation of influenza virus replication by splicing, and the bacteriophage T7 random walk. You can find TWiV #220 at www.microbe.tv/twiv.
On episode #30 of the science show This Week in Microbiology, Vincent, Elio, and Michael review how a toxin from Burkholderia pseudomallei inhibits protein synthesis, and the role of the gut microbiome in modulating insulin resistance in mice lacking an innate immune sensor. You can find TWiM #30 at microbeworld.org/twim.
A more rapid method than Western blot analysis to detect a specific protein in a cell, tissue, organ, or body fluid is enzyme-linked immunosorbent assay, or ELISA. This method, which does not require fractionation of the sample by gel electrophoresisis, is based on the property of proteins to readily bind to a plastic surface. To …
Let’s resume our discussion of the influenza virus genome. Last time we established that there are eight negative-stranded RNAs within the influenza virion, each coding for one or two proteins. Now we’ll consider how proteins are made from these RNAs. Figure 1 shows influenza RNA segment 2, which encodes two proteins: PB1 and PB1-F2. The …
