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Why doesn't HIV form plaques in any types of cells?
my remarks, notices, keywords:
viruses are the kings of evolution, diversity ?But viruses take ~10 hours per replication cycle, bakteria only 30min.
The virus-population is bigger, bakteria are larger, occupy more spacepolio : 30000 virues per cellquasispecies : every virus is different ? but at genbank we have identical genomesin different hosts
infection by different viruses ?but we don't see these. Why is there e.g. no H3N2,H1N1 combined transmittingquasispecies ? One virus seems to dominate, to succeed
genbank flu sequenes random, averages, just one particle – but we observe only35 mutations per year per genome or 0.03 mutations per replication cycle.
quasispecies different in mex ? so, it's coincidence that the sequences are identical ?Some mutants are in the quasispecies, but the majority is still identical
doesn't represent the population
one polio-mutation –> 3 times fewer errorsfewer errors are bad (why then DNA-viruses ?)5-azacytidine increases error ratesMuller's ratchet – I think I don't understand thiswhat's the sequence of bottleneck ratchet viruses ? where are they different ?herpes 200M years old 10^16 HIBs each daywe only know 1% of viruses
I'm not sure that anyone has tried very hard to develop a plaqueassay. The virus is cytopathic, yet the relevant cells for infectionare lymphocytes, which don't adhere to plastic dishes. Thereforedeveloping a plaque assay would not be straightforward.
http://latimesblogs.latimes.com/booster_shots/2…The researchers extracted white blood cells from all the subjects and exposed them to HIV in a culture dish. They reported in the journal BMC Immunology that HIV replication was slowed by about 80% in the cells from those who had received smallpox vaccination.
nice theme. but it takes a while to load
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