Virology lecture #22: Evolution

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  • Maggie Farhat 15 May 2010, 10:14 pm

    Why doesn't HIV form plaques in any types of cells?

  • gsgs 18 May 2010, 8:35 am

    my remarks, notices, keywords:

    viruses are the kings of evolution, diversity ?
    But viruses take ~10 hours per replication cycle, bakteria only 30min.

    The virus-population is bigger, bakteria are larger, occupy more space
    polio : 30000 virues per cell
    quasispecies : every virus is different ? but at genbank we have identical genomes
    in different hosts

    infection by different viruses ?
    but we don't see these. Why is there e.g. no H3N2,H1N1 combined transmitting
    quasispecies ? One virus seems to dominate, to succeed

    genbank flu sequenes random, averages, just one particle – but we observe only
    35 mutations per year per genome or 0.03 mutations per replication cycle.

    quasispecies different in mex ? so, it's coincidence that the sequences are identical ?
    Some mutants are in the quasispecies, but the majority is still identical

    doesn't represent the population

    one polio-mutation –> 3 times fewer errors
    fewer errors are bad (why then DNA-viruses ?)
    5-azacytidine increases error rates
    Muller's ratchet – I think I don't understand this
    what's the sequence of bottleneck ratchet viruses ? where are they different ?
    herpes 200M years old
    10^16 HIBs each day
    we only know 1% of viruses

  • profvrr 18 May 2010, 4:48 pm

    I'm not sure that anyone has tried very hard to develop a plaque
    assay. The virus is cytopathic, yet the relevant cells for infection
    are lymphocytes, which don't adhere to plastic dishes. Therefore
    developing a plaque assay would not be straightforward.

  • gsgs 19 May 2010, 6:44 am
    The researchers extracted white blood cells from all the subjects and exposed them to HIV in a culture dish. They reported in the journal BMC Immunology that HIV replication was slowed by about 80% in the cells from those who had received smallpox vaccination.

  • reverse phone lookup 17 November 2011, 9:18 pm

    nice theme. but it takes a while to load