Comments on: The D225G change in 2009 H1N1 influenza virus http://www.virology.ws/2010/03/18/the-d225g-change-in-2009-h1n1-influenza-virus/ About viruses and viral disease Sat, 11 Feb 2012 17:38:00 +0000 hourly 1 http://wordpress.org/?v=3.3.1 By: niman http://www.virology.ws/2010/03/18/the-d225g-change-in-2009-h1n1-influenza-virus/comment-page-1/#comment-21550 niman Thu, 25 Mar 2010 16:36:50 +0000 http://www.virology.ws/?p=2881#comment-21550 Once again, your comments on D225G are quite misleading. The WHO data you are citing came out last year (in December - the WER you linked was just a repeat of the 2009 report) and was thoroughly refuted by sequences that were published in January (Russia and Ukraine in particular). The Ukraine sequences were by Mill Hill (deposited at GISAID) and demonstrated D225G, D225N, or both in 27/37 autopsy lung samples, clearly showing clustering in time and space (all samples were collected over a 2-3 week period in Ukraine) and all samples were phylogenetically similar. The Ukraine sequences were DIRECT and did NOT involve lab isolation. Moreover, like Norway, virtually all samples with D225G were from fatal or severe cases. The Norway study also noted that in four of their patients with D225G, samples from the upper and lower respiratory tract were available, and D225G was detected in both. The Norway sequences were from H1N1 isolated in MDCK (mammalian) cells and did NOT include egg isolates.<br>However, mixed infections with D225G, D225N, and wild type are common, so detection can be quite dependent on sample source, collection time, and lab propagation.<br>There are 5 HA sequences from autopsy lung from the 1918/1919 pandemic. Two have D225G, including the only sample from 1919. <br>In 1918/1919 tens of millions died and D225G clear transmitted.<br>Recent papers show the similarities between the receptor binding domain of the 1918 H1N1 virus and 2009 pandemic H1N1.<br>The importance of D225G has been OBVIOUS since the release of the initial sequences from Ukraine in November. Moreover, the frequent asscociation with wild type and/or D225N allows for transmission (as seen in two family members in Italy). Once again, your comments on D225G are quite misleading. The WHO data you are citing came out last year (in December – the WER you linked was just a repeat of the 2009 report) and was thoroughly refuted by sequences that were published in January (Russia and Ukraine in particular). The Ukraine sequences were by Mill Hill (deposited at GISAID) and demonstrated D225G, D225N, or both in 27/37 autopsy lung samples, clearly showing clustering in time and space (all samples were collected over a 2-3 week period in Ukraine) and all samples were phylogenetically similar. The Ukraine sequences were DIRECT and did NOT involve lab isolation. Moreover, like Norway, virtually all samples with D225G were from fatal or severe cases. The Norway study also noted that in four of their patients with D225G, samples from the upper and lower respiratory tract were available, and D225G was detected in both. The Norway sequences were from H1N1 isolated in MDCK (mammalian) cells and did NOT include egg isolates.
However, mixed infections with D225G, D225N, and wild type are common, so detection can be quite dependent on sample source, collection time, and lab propagation.
There are 5 HA sequences from autopsy lung from the 1918/1919 pandemic. Two have D225G, including the only sample from 1919.
In 1918/1919 tens of millions died and D225G clear transmitted.
Recent papers show the similarities between the receptor binding domain of the 1918 H1N1 virus and 2009 pandemic H1N1.
The importance of D225G has been OBVIOUS since the release of the initial sequences from Ukraine in November. Moreover, the frequent asscociation with wild type and/or D225N allows for transmission (as seen in two family members in Italy).

]]> By: niman http://www.virology.ws/2010/03/18/the-d225g-change-in-2009-h1n1-influenza-virus/comment-page-1/#comment-21005 niman Thu, 25 Mar 2010 09:36:50 +0000 http://www.virology.ws/?p=2881#comment-21005 Once again, your comments on D225G are quite misleading. The WHO data you are citing came out last year (in December - the WER you linked was just a repeat of the 2009 report) and was thoroughly refuted by sequences that were published in January (Russia and Ukraine in particular). The Ukraine sequences were by Mill Hill (deposited at GISAID) and demonstrated D225G, D225N, or both in 27/37 autopsy lung samples, clearly showing clustering in time and space (all samples were collected over a 2-3 week period in Ukraine) and all samples were phylogenetically similar. The Ukraine sequences were DIRECT and did NOT involve lab isolation. Moreover, like Norway, virtually all samples with D225G were from fatal or severe cases. The Norway study also noted that in four of their patients with D225G, samples from the upper and lower respiratory tract were available, and D225G was detected in both. The Norway sequences were from H1N1 isolated in MDCK (mammalian) cells and did NOT include egg isolates.<br>However, mixed infections with D225G, D225N, and wild type are common, so detection can be quite dependent on sample source, collection time, and lab propagation.<br>There are 5 HA sequences from autopsy lung from the 1918/1919 pandemic. Two have D225G, including the only sample from 1919. <br>In 1918/1919 tens of millions died and D225G clear transmitted.<br>Recent papers show the similarities between the receptor binding domain of the 1918 H1N1 virus and 2009 pandemic H1N1.<br>The importance of D225G has been OBVIOUS since the release of the initial sequences from Ukraine in November. Moreover, the frequent asscociation with wild type and/or D225N allows for transmission (as seen in two family members in Italy). Once again, your comments on D225G are quite misleading. The WHO data you are citing came out last year (in December – the WER you linked was just a repeat of the 2009 report) and was thoroughly refuted by sequences that were published in January (Russia and Ukraine in particular). The Ukraine sequences were by Mill Hill (deposited at GISAID) and demonstrated D225G, D225N, or both in 27/37 autopsy lung samples, clearly showing clustering in time and space (all samples were collected over a 2-3 week period in Ukraine) and all samples were phylogenetically similar. The Ukraine sequences were DIRECT and did NOT involve lab isolation. Moreover, like Norway, virtually all samples with D225G were from fatal or severe cases. The Norway study also noted that in four of their patients with D225G, samples from the upper and lower respiratory tract were available, and D225G was detected in both. The Norway sequences were from H1N1 isolated in MDCK (mammalian) cells and did NOT include egg isolates.
However, mixed infections with D225G, D225N, and wild type are common, so detection can be quite dependent on sample source, collection time, and lab propagation.
There are 5 HA sequences from autopsy lung from the 1918/1919 pandemic. Two have D225G, including the only sample from 1919.
In 1918/1919 tens of millions died and D225G clear transmitted.
Recent papers show the similarities between the receptor binding domain of the 1918 H1N1 virus and 2009 pandemic H1N1.
The importance of D225G has been OBVIOUS since the release of the initial sequences from Ukraine in November. Moreover, the frequent asscociation with wild type and/or D225N allows for transmission (as seen in two family members in Italy).

]]> By: The D225G change in 2009 H1N1 influenza virus | H1N1INFLUENZAVIRUS.US http://www.virology.ws/2010/03/18/the-d225g-change-in-2009-h1n1-influenza-virus/comment-page-1/#comment-20985 The D225G change in 2009 H1N1 influenza virus | H1N1INFLUENZAVIRUS.US Tue, 23 Mar 2010 06:34:39 +0000 http://www.virology.ws/?p=2881#comment-20985 [...] to cause lower respiratory tract disease. The D225G change might be … The rest is here: The D225G change in 2009 H1N1 influenza virus Posted in H1N1 Flu, H1N1 Influenza Virus, Influenza Virus. Tags: 2009-swine-origin, ability, [...] [...] to cause lower respiratory tract disease. The D225G change might be … The rest is here: The D225G change in 2009 H1N1 influenza virus Posted in H1N1 Flu, H1N1 Influenza Virus, Influenza Virus. Tags: 2009-swine-origin, ability, [...]

]]> By: uberVU - social comments http://www.virology.ws/2010/03/18/the-d225g-change-in-2009-h1n1-influenza-virus/comment-page-1/#comment-20948 uberVU - social comments Fri, 19 Mar 2010 06:18:38 +0000 http://www.virology.ws/?p=2881#comment-20948 <strong>Social comments and analytics for this post...</strong> This post was mentioned on Twitter by profvrr: The D225G change in 2009 H1N1 influenza virus http://bit.ly/bSUKHt at virology blog #h1n1 #swineflu... Social comments and analytics for this post…

This post was mentioned on Twitter by profvrr: The D225G change in 2009 H1N1 influenza virus http://bit.ly/bSUKHt at virology blog #h1n1 #swineflu…

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