Comments on: Rhinovirus and zinc part 4: cell toxicity http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/ About viruses and viral disease Sat, 11 Feb 2012 17:38:00 +0000 hourly 1 http://wordpress.org/?v=3.3.1 By: BM http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-21703 BM Tue, 05 Jan 2010 02:07:48 +0000 http://www.virology.ws/?p=2522#comment-21703 Is it worth trying an agar overlay? You start with agar containing the Zn you want....add another plain agar overlay and then your Hela cells...which you can infect with virus. Is it worth trying an agar overlay? You start with agar containing the Zn you want….add another plain agar overlay and then your Hela cells…which you can infect with virus.

]]> By: BM http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19549 BM Mon, 04 Jan 2010 18:07:48 +0000 http://www.virology.ws/?p=2522#comment-19549 Is it worth trying an agar overlay? You start with agar containing the Zn you want....add another plain agar overlay and then your Hela cells...which you can infect with virus. Is it worth trying an agar overlay? You start with agar containing the Zn you want….add another plain agar overlay and then your Hela cells…which you can infect with virus.

]]> By: Rhinovirus and zinc part 5: Magnesium is not the culprit http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19333 Rhinovirus and zinc part 5: Magnesium is not the culprit Wed, 16 Dec 2009 17:36:20 +0000 http://www.virology.ws/?p=2522#comment-19333 [...] know that I’ve been trying to determine why concentrations of the salt higher than 0.1 mM are toxic to HeLa cells. I have found that 0.1 mM ZnCl2 does inhibit rhinovirus plaque formation but not sufficiently to be [...] [...] know that I’ve been trying to determine why concentrations of the salt higher than 0.1 mM are toxic to HeLa cells. I have found that 0.1 mM ZnCl2 does inhibit rhinovirus plaque formation but not sufficiently to be [...]

]]> By: profvrr http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19268 profvrr Sun, 13 Dec 2009 00:12:48 +0000 http://www.virology.ws/?p=2522#comment-19268 Hadn't seen that paper. If Ca reduces cytotoxicity by blocking<br>channels shared by Zn then it might block the antiviral action of the<br>metal. But I'll give it a try anyway - easy enough to do. Thanks for<br>bringing it up. Hadn't seen that paper. If Ca reduces cytotoxicity by blocking
channels shared by Zn then it might block the antiviral action of the
metal. But I'll give it a try anyway – easy enough to do. Thanks for
bringing it up.

]]> By: iayork http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19259 iayork Sat, 12 Dec 2009 01:14:41 +0000 http://www.virology.ws/?p=2522#comment-19259 Vincent - have you seen Chemico-Biological Interactions 69:279-291 (1989) (Cytotoxicity of zinc in vitro)? Perhaps increasing Ca might be worth a try, if you haven't already. From the abstract -<br><br>"The proliferation of B16, HeLa and I-221 cell lines was inhibited by 1.25 × 10−4, 1.50 × 10−4 and 1.50 × 10−4 mol/l Zn2+, respectively. The free radical scavengers, methimazole and ethanol, did not suppress the toxicity of Zn2+, neither did superoxide dismutase or catalase. The addition of the chelating agent EDTA reduced the zinc cytotoxicity. It was possible to suppress the cytotoxicity of zinc by increasing the concentration of either Fe2+ or Ca2+ but not Mg2+, which suggests that a prerequisite for the toxic action of zinc is entry into cells using channels that are shared with iron or calcium. This view was supported by experiments in which transferrin intensified the cytotoxic action of zinc in serum-free medium." Vincent – have you seen Chemico-Biological Interactions 69:279-291 (1989) (Cytotoxicity of zinc in vitro)? Perhaps increasing Ca might be worth a try, if you haven't already. From the abstract -

“The proliferation of B16, HeLa and I-221 cell lines was inhibited by 1.25 × 10−4, 1.50 × 10−4 and 1.50 × 10−4 mol/l Zn2+, respectively. The free radical scavengers, methimazole and ethanol, did not suppress the toxicity of Zn2+, neither did superoxide dismutase or catalase. The addition of the chelating agent EDTA reduced the zinc cytotoxicity. It was possible to suppress the cytotoxicity of zinc by increasing the concentration of either Fe2+ or Ca2+ but not Mg2+, which suggests that a prerequisite for the toxic action of zinc is entry into cells using channels that are shared with iron or calcium. This view was supported by experiments in which transferrin intensified the cytotoxic action of zinc in serum-free medium.”

]]> By: profvrr http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19257 profvrr Fri, 11 Dec 2009 23:12:34 +0000 http://www.virology.ws/?p=2522#comment-19257 Yes, I think that's a very good idea. I'm sure I could derive cells<br>resistant to higher concentrations of ZnCl2. I'll give it a try,<br>starting Monday! Yes, I think that's a very good idea. I'm sure I could derive cells
resistant to higher concentrations of ZnCl2. I'll give it a try,
starting Monday!

]]> By: BN http://www.virology.ws/2009/12/11/rhinovirus-and-zinc-part-4-cell-toxicity/comment-page-1/#comment-19254 BN Fri, 11 Dec 2009 22:48:31 +0000 http://www.virology.ws/?p=2522#comment-19254 Since these cells grow fine in 0.1mM ZnCl2, will this work: passaging these cells in 0.1mM ZnCl2 for certain period so that cells become adapted to 0.1mM salt concentration then increasing the salt concentration above 0.1mM? Since these cells grow fine in 0.1mM ZnCl2, will this work: passaging these cells in 0.1mM ZnCl2 for certain period so that cells become adapted to 0.1mM salt concentration then increasing the salt concentration above 0.1mM?

]]>