Comments on: Influenza microneutralization assay

By: reverse phone lookup

reverse phone lookup — Thu, 17 Nov 2011 21:23:00 +0000

Looks like you’ve done your research very well.

By: Changsc

Changsc — Fri, 06 May 2011 02:07:00 +0000

The MN protocol used in CDC is a traditional neutrlization test combined with ELISA method. However, the ELISA procedure is highly dependent on antibody quality. I don’t know how to get the first antibody (anti-NP antibody) if I want to set up this platform in our Lab. Only CDC has this standard NP antibody. So… Can I just use the traditional NT based on crystal violet staining?

By: Scientist

Scientist — Wed, 02 Jun 2010 21:04:38 +0000

It seems to me these assays confound the neutralization abilities of particular IgG antibodies and their concentration in serum. There's also the question of how effective antibody concentrations map to the kinetics of infection. Do standing (“equilibrium”) concentrations correlate linearly with response upon challenge?

By: Headless HA: Universal influenza vaccine?

Headless HA: Universal influenza vaccine? — Fri, 28 May 2010 21:04:04 +0000

[...] encouraging, they are not unequivocally positive. For example, it was not possible to demonstrate neutralizing antibodies in sera from mice immunized with headless H1 or H3 HA proteins. Furthermore, the ability of [...]

By: profvrr

profvrr — Mon, 16 Nov 2009 06:31:38 +0000

Thank you!

By: Ada

Ada — Mon, 16 Nov 2009 05:00:00 +0000

That was so clearly and simply explained. Thanks so much! I was just trying to learn it from listening to a recorded lecture and looking at black and white badly xeroxed pictures.

By: profvrr

profvrr — Sun, 15 Nov 2009 22:31:38 +0000

Thank you!

By: Ada

Ada — Sun, 15 Nov 2009 21:00:00 +0000

That was so clearly and simply explained. Thanks so much! I was just trying to learn it from listening to a recorded lecture and looking at black and white badly xeroxed pictures.

By: SCIENCEPODCASTERS.ORG » This Week in Virology #50: XMRV

SCIENCEPODCASTERS.ORG » This Week in Virology #50: XMRV — Sun, 20 Sep 2009 14:08:34 +0000

[...] antibodies against influenza 2009 H1N1 strain Explanation of hemagglutination-inhibition and microneutralization assays FDA approves influenza 2009 H1N1 vaccine XMRV is present in malignant prostatic epithelium [...]

By: TWiV 50: XMRV

TWiV 50: XMRV — Sun, 20 Sep 2009 14:04:32 +0000

By: profvrr

profvrr — Thu, 28 May 2009 16:46:34 +0000

You are right, there are now many other cells that are used in
research, many of which have the correct number of chromosomes. But
HeLa cells continue to be used because they are very easy to grow, and
last nearly forever.

By: Teeny

Teeny — Thu, 28 May 2009 16:05:04 +0000

I was so suprised when my lecturer explained to us that labs still use these HeLa cells even though they aren't even human anymore. I've been wondering since, why don't they get a 'fresh' batch of transformed cells from a more recent donor? Surely cells with the correct chromosome number is the more sensible choice?