Comments on: Infectious DNA clones

By: reverse phone lookup

reverse phone lookup — Thu, 17 Nov 2011 21:26:00 +0000

Looks like you’ve done your research very well.

By: Thirty years of infectious enthusiasm

Thirty years of infectious enthusiasm — Wed, 03 Aug 2011 17:35:01 +0000

[...] poliovirus RNA, extracted from the virion, is also infectious when introduced into such cells by transfection. What would happen if we transfected pVR106 into cells? I asked David if I should first add a [...]

By: XMRV infection of Rhesus macaques

XMRV infection of Rhesus macaques — Fri, 18 Feb 2011 19:08:31 +0000

[...] XMRV – a good amount of virus, to ensure infection. The virus used, VP62, was produced by transfecting cells with cloned viral DNA isolated from human [...]

By: Bats harbor many viral sequences

Bats harbor many viral sequences — Wed, 03 Nov 2010 09:09:05 +0000

[...] require construction of complete DNA copies of viral genomes and recovery of infectious viruses by transfection of cells in [...]

By: Beyonce Knowles Sunglasses

Beyonce Knowles Sunglasses — Fri, 20 Feb 2009 18:23:50 +0000

That's bad an infectious clone. Well DNA sometimes cause bad.

By: QMS

QMS — Fri, 20 Feb 2009 10:23:50 +0000

That's bad an infectious clone. Well DNA sometimes cause bad.

By: profvrr

profvrr — Fri, 13 Feb 2009 11:29:15 +0000

What I did not mention in the post was that many labs tried for years
to produce infectious DNA of reovirus. All kinds of reasons for why it
didn't work were set forth. In the end, it turned out that the
sequences were wrong. Kobayashi et al sequenced many clones until they
got it right, and the result was infectivity. So make sure your
sequences are correct....sequence multiple independent clones for each
dsRNA segment and arrive at a consensus. Good luck, and glad you enjoy
virology blog.

By: Michael Day

Michael Day — Fri, 13 Feb 2009 10:35:23 +0000

I am just beginning to anticipate needing to produce infectious clones of reovirus and rotavirus, and I had not even begun to look at the available literature. The Kobayashi reference is going to help immensely, I predict!
(Just found your blog and I have enjoyed reading it…)